Named the Lennoxlove Amen glass, its provenance traces back to the Lords Blantyre of Lennoxlove, Haddington and their successors, the Bairds of Newbyth.
It had passed through Christie's in 1947 before the late glass collector Edward V Phillips acquired it in 1986 from Asprey.
On a plain drawn stem and folded foot, the 7in (17.5cm) glass was engraved to the body with a crown, cipher, a small 8 and two verses of the Jacobite anthem fully attributed to the expert diamond point engraver, Sir Robert Strange (1721-92).
Bidding on November 6 quickly rose beyond the pre-sale estimate of £20,000-30,000 before the hammer fell at £43,000 (plus 17.5% buyer's premium) to a telephone bidder.
The glass eclipsed two others sold in the late 1990s at Christie's; the Ker Amen glass and the Ogilvy of Inshewan Amen glass, which sold for £34,500 and £35,600 respectively.